| Registro completo |
| Provedor de dados: |
Arq. Bras. Med. Vet. Zootec.
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| País: |
Brazil
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| Título: |
Validation of an HPLC-UV method for the identification and quantification of bioactive amines in chicken meat
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| Autores: |
Assis,D.C.S.
Menezes,L.D.M.
Lima,A.L.
Klein,R.W.T.
Heneine,L.G.D.
Lara,L.J.C.
Teixeira,L.V.
Ornellas,C.B.D.
Cançado,S.V.
Figueiredo,T.C.
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| Data: |
2016-06-01
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| Ano: |
2016
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| Palavras-chave: |
Validation
Chicken meat
Bioactive amines
HPLC-UV
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| Resumo: |
ABSTRACT A high-performance liquid chromatography with ultraviolet detection (HPLC-UV) method was validated for the study of bioactive amines in chicken meat. A gradient elution system with an ultraviolet detector was used after extraction with trichloroacetic acid and pre-column derivatization with dansyl chloride. Putrescine, cadaverine, histamine, tyramine, spermidine, and spermine standards were used for the evaluation of the following performance parameters: selectivity, linearity, precision, recovery, limits of detection, limits of quantification and ruggedness. The results indicated excellent selectivity, separation of all amines, a coefficient of determination greater than 0.99 and recovery from 92.25 to 102.25% at the concentration of 47.2mg.kg-1, with a limit of detection at 0.3mg.kg-1 and a limit of quantification at 0.9mg.kg-1 for all amines, with the exception of histamine, which exhibited the limit of quantification, of 1mg.kg-1. In conclusion, the performance parameters demonstrated adequacy of the method for the detection and quantification of bioactive amines in chicken meat.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000300805
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| Editor: |
Universidade Federal de Minas Gerais, Escola de Veterinária
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| Relação: |
10.1590/1678-4162-8668
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| Formato: |
text/html
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| Fonte: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia v.68 n.3 2016
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| Direitos: |
info:eu-repo/semantics/openAccess
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